Multi-location field testing new environmentally friendly, high yielding, low phytate soybean lines developed via molecular breeding
Willette, A., J. Boehm, Jr., F. R. Walker, H. Bhandari, D. A. Kopsell, K. Bilyeu, and V. R. Pantalone.  2014.  Crop Science, ASA-CSSA-SSA International Meeting Abstracts. November 2-5, 2014. Long Beach, CA.

High protein soymeal is a primary soybean use and staple food of livestock and fish. The storage form of phosphorus (P) in the seed is phytic acid, or phytate (myo-inositol 1,2,3,4,5,6 hexakisphosphate) which binds and chelates many key nutritional cations forming stable salts that are non-digestible. Livestock waste laden with phytate P is an environmental concern, and a source of nonpoint P pollution causing accelerated eutrophication and surface water quality deterioration. The primary objective of this research was the development of a commercially acceptable low phytate (LP) soybean line which would enhance nutritional qualities of soymeal for livestock and sustainably lessen P pollution from animal agricultural waste runoff. In 2012, a field trial using a randomized complete block design (RCBD) with two replications was grown at two locations in two row plots to evaluate the agronomic performance of 10 BC5 LP lines in comparison to their parents 5601T, TN09-239, and high yield check cultivars; the study was repeated in 2013, adding two additional BC5 lines which were evaluated in four row plots in two locations and in two row plots in a third location with three replications per location. Data analyzed using SAS version 9.3 revealed that the yields of four BC5F3:5 LP lines, 56CX-1273 (4107 Kg ha-1), 56CX-1274 (4137 Kg ha-1 ), 56CX-1277 (4112 Kg ha-1) and 56CX-1282 (4160 Kg ha-1), were not significantly different (p>0.05) than that of 5601T (4227 Kg ha-1) and also were numerically better than the yield of current USDA Maturity Group V check 'Osage' (4089 Kg ha-1). These results indicate that LP trait introgression to those four BC5 LP lines did not have detrimental effects on yield and one of the lines may be considered for a cultivar or germplasm release.