Breeding for improved oil quality in high yielding soybean lines using molecular markers for selecting fAD3A and FAD3C mutant alleles
A. Willette, H. Bhandari, C. E. Sams, F. Chen, V. R. Sykes, C. Smallwood, and V. R. Pantalone.  2017.  World Soybean Research Conference 10, Poster completed and displayed in Plant Biotechnology Bldg. Conference cancelled due to Hurricane Irma.

Soybean oil is a major commodity in the US with practical uses for both food and industrial products. High quantities of linolenic fatty acid (FA) in soybean oil causes rapid rancidity. To accommodate the need for a more stable oil, soybean breeders have identified alleles FAD3A, and FAD3C, which regulate linolenic FA (18:3). New CODEX standards for improved oil necessitate a decreased in linolenic FA to < 3%. Using single nucleotide polymorphism’s (SNP’s) and gas chromatography to identify low linolenic (LL) FA soybean lines, soybean breeders can produce soybeans with more stable soybean oil. In 2016 we performed a multi-location yield study comparing four genotypic classes of F7 near isogenic lines with parents and high yielding checks. The four genotypic classes assessed as lines were as follows; mutant/mutant (ffgg), mutant/wild-type (ffGG), wild-type/mutant (FFgg) and wild-type/wild-type (FFGG). This study was conducted with two replications of two row plots in a randomized complete block design (RBD) at East Tennessee Research and Education Center and Highland Rim Research and Education Center. We found that no yield drag was associated with any of the LL lines assessed when compared to checks and parents. The mean FA of the mutant line ffgg genotypic class was 2.6% with 0.14 SD when tested at α=0.05 level of statistical significance. The results of this study indicate that through SNP analysis we can determine that the FA concentration for developing low linolenic soybean lines with less than 3% linolenic FA.